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Isingiro District is located in the Ankole sub region in western Uganda and it was part of Mbarara district until it achieved district status in 2005. It is bordered by Mbarara to the North West, Ntungamo to the West, Kiruhura to the North, Rakai to the East and Tanzania to the South. The district hosts the oldest refugee settlement in Africa; Nakivale Refugee Settlement, as well as Oruchinga refugee settlement, both settlements were established in the late 1950’s. 

The total population stood at 486,360 people, as of 2014 and the major language is runyankole.

Isingiro district has the highest plantain banana (gonja) production in the country and also, as of 2015, the district emerged as the leading producer of matooke in Uganda.

Tourists hotspot in Isingiro districts is Lake Mburo National Park, which hosts Zebras, hippopotamus, impala, warthog, common eland, African buffalo and over 300 bird species.